Template-ready PCR method for detection of human telomerase reverse transcriptase mRNA in sputum
نویسندگان
چکیده
منابع مشابه
detection of telomerase reverse transcriptase mrna in peripheral blood mononuclear cells of patients with liver failure
results the median level of pbmcs htert mrna in survivors increased with treatment time and was significantly higher than the corresponding level in non-survivors after 14 days of treatment (p < 0.001). subgroup analyses showed that the levels of pbmcs htert mrna were remarkably higher in patients with acute-on-chronic liver failure than in those with chronic liver failure (p < 0.05). in patien...
متن کاملUsefulness of Immunological Detection of the Human Telomerase Reverse Transcriptase
OBJECTIVE Recent years have seen a considerable wealth of studies conducted on the potential usefulness of telomerase determination in diagnosis, prognosis and targeted cancer therapy. The frequently used Telomeric Repeat Amplification Protocol assay suffers from some drawbacks, the most important being the rate of false positives. In situ analysis using well characterised antibodies directed a...
متن کاملDetection of Telomerase Reverse Transcriptase mRNA in Peripheral Blood Mononuclear Cells of Patients With Liver Failure
BACKGROUND Telomerase activity is closely associated with the expression of human telomerase reverse transcriptase (hTERT) mRNA; although it can be induced in hepatocytes during liver regeneration, its dynamic change in patients with liver failure has remained unclear. OBJECTIVES We investigated the variation and significance of hTERT mRNA expression in peripheral blood mononuclear cells (PBM...
متن کاملHuman telomerase reverse transcriptase binds to a pre-organized hTR in vivo exposing its template
Telomerase is a specialized reverse transcriptase that is responsible for telomere length maintenance. As in other organisms, the minimal components required for an active human telomerase are the template-providing telomerase RNA (hTR) and the enzymatic entity telomerase reverse transcriptase (hTERT). Here, we explored the structure of hTR and the hTERT-induced conformational changes within hT...
متن کاملReal-time quantification of human telomerase reverse transcriptase mRNA in tumors and healthy tissues.
BACKGROUND Expression of the hTERT gene, which codes for the catalytic subunit of telomerase, is associated with malignancy. We recently developed a real-time reverse transcription-PCR assay, based on TaqMan technology, for accurate and reproducible determination of hTERT mRNA expression (Lab Investig 1999;79:911-2). This method may be of interest for molecular tumor diagnostics in tissues and ...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2019
ISSN: 0003-2697
DOI: 10.1016/j.ab.2019.04.008